Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

B Cell Activation and Differentiation

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

LCK-targeting molecular glues overcome resistance to inhibitor-based therapy in T-cell acute lymphoblastic leukemia.

Blood·2026

Same author

Presentation and management of pediatric T-cell acute lymphoblastic leukemia with mediastinal mass and hyperleukocytosis.

Haematologica·2026

Same author

Characterization of Genetic Etiologic Factors for Pediatric Acute Lymphoblastic Leukemia in Large Childhood Cancer Survivorship Cohorts.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2026

Same author

Protein phosphatase 2A regulates senescence and immunogenicity in medulloblastoma models.

The Journal of clinical investigation·2026

Same author

Genomic Subtype Influences BH3 Mimetic Drug Sensitivity and Synergy with Cytotoxic Chemotherapeutics in T-cell Acute Lymphoblastic Leukemia.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026

Same author

Chlorosyl Nitrite (OClNO): An Elusive Intermediate in the Photochemistry of Nitryl Chloride.

Journal of the American Chemical Society·2026

Same journal

Protocol for semi-automatic quantitative bioimaging analysis of synapse loss.

STAR protocols·2026

Same journal

Protocol for integrated ubiquitination analysis of in vitro E3 ligase-DUB regulation and in vivo ubiquitin chain linkage characterization.

STAR protocols·2026

Same journal

Protocol for constructing multi-ancestry polygenic models using S4-Multi.

STAR protocols·2026

Same journal

Protocol for inducing deep vein thrombosis in C57BL/6J mice using the inferior vena cava stenosis model.

STAR protocols·2026

Same journal

Overcoming the challenges of genome-editing essential genes.

STAR protocols·2026

Same journal

Protocol for longitudinal two-photon calcium imaging and holographic optogenetic manipulation to investigate memory in mice.

STAR protocols·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 6, 2026

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

Protocol for predicting the single-cell network-based gene activity landscape during human B cell development.

Xin Huang¹, Xuetong Hou², Yizhen Li³

¹Precision Research Center for Refractory Diseases, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China.

|February 8, 2025

Summary

This summary is machine-generated.

This study introduces a new protocol for analyzing human B cell development using gene regulatory networks. It helps clarify B cell subtypes and their biological characteristics during development.

Keywords:

bioinformatics immunology single Cell systems biology

More Related Videos

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

Related Experiment Videos

Last Updated: May 6, 2026

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

Area of Science:

Immunology
Computational Biology
Genomics

Background:

Human B cell classification faces challenges due to heterogeneity.
Distinguishing between B cell subpopulations is difficult.

Purpose of the Study:

To present a protocol for constructing gene regulatory networks and gene activity landscapes for human B cell developmental stages.
To elucidate the biological characteristics of human B cell development.

Main Methods:

Acquisition of bone marrow data.
Single-cell downstream analysis.
Network-based analysis using SJARACNe, NetBID2, and scMINER algorithms.

Main Results:

The protocol provides a method to construct gene regulatory networks for human B cell development.
It enables the creation of gene activity landscapes.
Biological characteristics of developmental stages are elucidated.

Conclusions:

The developed protocol offers a robust framework for studying human B cell development.
It addresses inconsistencies in B cell classification and subpopulation identification.
This approach enhances understanding of B cell biology.