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Updated: May 12, 2026

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Published on: January 3, 2012
Dual-mode detection of Ochratoxin A based on silver nanocluster and phosphate
Xin Yang1, Yuanyuan Wang1, Xiaowei Chen2
1Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou 434025, People's Republic of China.; College of Animal Science and Technology, Yangtze University, Jingzhou 434025, People's Republic of China.
Abstract:
Ochratoxin A (OTA) is widely present in the environment and has great harm to human safety. Therefore, a simple and sensitive method for OTA detection is urgently needed. Herein, a fluorescence and colorimetric dual-mode immunoassay based on glutathione silver nanocluster (GSH-AgNCs) and phosphate colorimetry was established. For fluorescence mode, the pyrophosphate ion (PPi) can reduce the fluorescence of GSH-AgNCs@Al3+ by chelating Al3+. Meanwhile, the fluorescence can be recovered when PPi is hydrolyzed to phosphate ion (Pi) by alkaline phosphatase (ALP.) For colorimetric mode, Pi can be quantitatively analyzed by MoSb colorimetric method. The linear range of fluorescence mode was 1.25-35 ng/mL with limit of detection (LOD) of 0.54 ng/mL. For colorimetric mode, the linear range and LOD was 6.25-250 ng/mL and 3.13 ng/mL. The detection limits are much lower than the European Union's regulations. And this dual-mode immunoassay showed a satisfactory recovery rate and specificity in detecting real samples.

