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Updated: May 28, 2025
Human Neural Organoids for Studying Brain Cancer and Neurodegenerative Diseases
Published on: June 28, 2019
Surface-enhanced Raman scattering probes based on sea urchin-like Bi2S3@Co3O4 composite for the detection of dopamine
Qinyan Cai1, Min Chen1, Zhendi Wang1
1Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Dongchuan Road 500, Shanghai 200241, China.
Abstract:
Dopamine (DA) is a significant neurotransmitter involved in various functions of the central nervous system, containing the regulation of motor functions, arousal, motivation and reward processing. Therefore, precise measurement of DA concentrations is vital for studying different dopaminergic neural pathways and for the diagnosis of neurological disorders. Herein, we developed a new semiconductor surface-enhanced Raman spectroscopic (SERS) platform designed for the real-time monitoring DA release from live cells with high selectivity and sensitivity. The sea urchin-like Bi2S3@Co3O4 composite substrate exhibited not only a wide linear range from 1.00 to 1.00 × 103 nmol/L but also a limit of detection (LOD) as low as 0.92 nmol/L for Methylene Blue (MB) molecules. In addition, we monitored DA release during the differentiation of neural stem cells (NSCs) into dopaminergic neurons by virtue of our constructed SERS platform in a nondestructive and in situ manner. Consequently, the developed SERS platform facilitates the direct detection of exocytotic DA from neuronal cells, enabling real-time assessment of cell viability in models of neurodegenerative disease-related cellular damage.
