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Updated: May 28, 2025

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
Published on: September 20, 2016
Short and High-Yielding Synthesis of a Minimalist Diazirine-Alkyne Photo-Cross-Linker and Photoaffinity Labeling
Dare E George1,2, Miracle O Olatunde2, Jetze J Tepe2
1Department of Chemistry, Michigan State University, East Lansing, Michigan 48823, United States.
Abstract:
Minimalist photo-cross-linker 1 and its derivatives are extensively utilized in photoaffinity labeling studies. However, obtaining compound 1 in high yield has traditionally required a lengthy synthetic process. In this paper, we present a concise and efficient method to synthesize 1 in just four steps, leveraging the "Normant reagent" as a pivotal component in our strategy. Additionally, we extended our synthetic approach to generate new derivatives of the fully functionalized diazirine tag, providing versatile handles for coupling with other small molecules. This work provides a quick and high-yielding approach to access photo-cross-linker 1 compared to previously reported approaches.
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