Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mechanistic model for HEK293 viral vector processes and its application in a digital shadow framework.

Bioprocess and biosystems engineering·2026

Same author

Service Evaluation of a "Straight to Scan" Pathway for Neck Lumps Referred as Urgent Suspected Head and Neck Cancer.

Laryngoscope investigative otolaryngology·2026

Same author

Advancing Raman Calibration: Automated Data Generation, Monitoring, and Control in Multi-Parallel Perfusion Mini Bioreactors.

Biotechnology journal·2026

Same author

Scalable CAR-T production in a 2-litre perfusion stirred-tank bioreactor with automated harvesting and scale-down model characterisation.

Frontiers in bioengineering and biotechnology·2026

Same author

Lab-Scale Continuous Biomanufacturing: A Tool for Process Development With Adaptive Strategies for Capture and Virus Inactivation.

Biotechnology and bioengineering·2026

Same author

Autonomous Spatiotemporal Regulation of Reversible Hydrogel Actuators by Chemical Reaction Networks.

Advanced materials (Deerfield Beach, Fla.)·2025

Same journal

Filament Extrusion-Based Conductive TPU Composite Scaffolds Enable Superior Neuronal Growth and Synaptic Maturation In Vitro.

Engineering in life sciences·2026

Same journal

Less Genome, More Gain: Genome Reduction Enhances Transaminase-Producing <i>E. coli</i> in a Scale-Down Bioreactor.

Engineering in life sciences·2026

Same journal

Design and Characterization of a 3D-Printable Membrane Aeration Module for Small-Scale Bioprocess Prototyping.

Engineering in life sciences·2026

Same journal

Molecular Pharming: Advances, Applications, and Future Prospects in Biotechnology and Medicine.

Engineering in life sciences·2026

Same journal

Topic: Continuous Enzymatic Peracids Synthesis in Pickering Emulsions: Influence of Nanoparticles Modification, Aqueous Phase Composition and Operational Parameters.

Engineering in life sciences·2026

Same journal

Optimized Bioconversion of Naringenin to Hesperetin in <i>Escherichia coli</i> With Halide Methyltransferase-Mediated <i>S</i>-Adenosylmethionine Regeneration.

Engineering in life sciences·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 28, 2025

Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis

Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis

Published on: May 5, 2023

Real-Time VCC Monitoring and Forecasting in HEK-Cell-Based rAAV Vector Production Using Capacitance Spectroscopy.

Rafael Machleid¹, Suneetha Nunna², Ajith George¹

¹Sartorius Stedim Biotech GmbH Advanced Analytics & Spectroscopy, Embedded Hardware Göttingen Germany.

Engineering in Life Sciences

|February 10, 2025

Summary

This summary is machine-generated.

This study introduces real-time monitoring of viable cell concentration (VCC) in human embryonic kidney (HEK293) cell cultures for recombinant adeno-associated virus (rAAV) production. Inline capacitance probes and predictive models enable accurate VCC tracking and transfection time forecasting.

More Related Videos

Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors

Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors

Published on: April 5, 2024

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

Published on: June 30, 2021

Related Experiment Videos

Last Updated: May 28, 2025

Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis

Monitoring Changes in Human Umbilical Vein Endothelial Cells upon Viral Infection Using Impedance-Based Real-Time Cell Analysis

Published on: May 5, 2023

Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors

Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors

Published on: April 5, 2024

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

Published on: June 30, 2021

Area of Science:

Biotechnology
Bioprocess Engineering
Cell Culture Technology

Background:

Robust cultivation of human embryonic kidney (HEK293) cells is critical for high-quality recombinant adeno-associated virus (rAAV) vector production.
Monitoring viable cell concentration (VCC) is essential for upstream process management and ensuring optimal viral vector yield.
Process Analytical Technology (PAT) tools, such as inline capacitance probes, offer real-time VCC measurement capabilities.

Purpose of the Study:

To develop and apply a method for real-time VCC monitoring in HEK293-based rAAV vector production.
To integrate inline capacitance probes and predictive models into a bioprocess control system.
To enable real-time forecasting of the optimal transfection time point.

Main Methods:

Utilized BioPAT Viamass capacitance probes to record data from 10 L rAAV-8 batches across a frequency range of 50 kHz-20 MHz.
Developed linear single-frequency and orthogonal partial least square (OPLS) multifrequency models for VCC prediction based on capacitance data.
Deployed predictive models inline and integrated predictions into BioPAT MFCS software for real-time monitoring and forecasting.

Main Results:

Successfully implemented inline capacitance probes for real-time VCC monitoring in HEK293 cell cultures.
Developed and validated both linear and OPLS models for accurate VCC prediction.
Integrated VCC monitoring with an exponential cell growth model for precise, real-time forecasting of the transfection time point.

Conclusions:

This work represents the first inline deployment of VCC and Time-Till-Transfection predictive models in a bioprocess control system for HEK-cell-based rAAV production.
Real-time monitoring and forecasting of VCC and transfection timing enhance process control and optimize viral vector production.
Inline capacitance technology offers a powerful PAT tool for improving the efficiency and quality of biopharmaceutical manufacturing.