The catecholamine response to graded high-altitude flight in yellow-rumped warblers (Setophaga coronata)

Chronic exposure to low oxygen (hypoxia) leads to amplification of the hypoxic chemoreflex, increasing breathing and sympathetic nervous system (SNS) activation. Prolonged SNS activation redistributes blood to hypoxia-sensitive tissues away from muscles. Recent tracking studies have shown that migratory songbirds can fly 5,000 m or higher above sea level, leading us to hypothesize that migratory birds may have a blunted hypoxic chemoreflex to maintain blood flow to muscles during migratory flight at high altitudes. To test this hypothesis, we used a hypobaric wind tunnel and measured circulating plasma catecholamines after maximal altitude flight, flight at 75% of maximal altitude, flight at ground level (∼250 m), and after rest at 75% of maximal altitude and ground level in migratory myrtle yellow-rumped warblers (Setophaga coronata). Yellow-rumped warblers were capable of flying above 4,000 m simulated altitude above sea level (average maximum altitude of ∼3,600 m) and would maintain flights at 75% of individual maximum altitudes (∼2,700 m). Circulating dopamine and noradrenaline were similar between resting and flight conditions at ground level and with exposure to 75% of maximal altitude, whereas adrenaline significantly increased with flight, but did not change further with flight at 75% of maximal altitude. In contrast, both adrenaline and noradrenaline concentrations increased after maximum altitude flights compared with 75% and ground-level flights. Our findings show that exercise increases plasma adrenaline in migratory songbirds and suggest that warblers flying at high altitudes below their maximum altitude may be minimally hypoxic, allowing them to maintain oxygen transport to flight muscles.NEW & NOTEWORTHY Yellow-rumped warblers, a small songbird that conducts migratory flights, were found to fly to altitudes above 4,000 m above sea level in simulated flights using a hypobaric wind tunnel. Circulating adrenaline suggests that warblers flying at 75% of individual maximum altitudes are not experiencing arterial hypoxia, allowing them to maintain aerobically demanding migratory flight at high altitudes.