Related Experiment Video
Updated: May 28, 2025

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures
Published on: December 22, 2014
Vulnerability of the Nrl-/- Cone-Dominant Retina to Endoplasmic Reticulum Stress
Angela Galdamez1,2,3, Masako Le1,2, Wei-Chieh Jerry Chiang4
1Department of Ophthalmology, Stanford University, Palo Alto, CA, USA.
Abstract:
Neural retina leucine zipper (NRL) is essential for the development of rods. The Nrl knockout (Nrl-/-) mouse develops a cone-rich retina which facilitates investigations of cone biology. Previously, we identified the endoplasmic reticulum (ER) stress response gene ATF6 as a cause of cone dysfunction in people. Here, we investigated Atf6's influence on mouse cones in the cone-dominant Nrl-/- mouse. We find that cone development appears unaffected, but the cone-dominant Nrl-/- retina is highly vulnerable to ER stress-induced damage.

