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Updated: May 28, 2025
OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides
Published on: June 20, 2010
Ultrasonic/compound enzyme extraction, comparative characterisation and biological activity of Lonicera macranthoides
Mengdie Ren1, Yiru Hou1, Danli Peng1
1School of Pharmacy, Zunyi Medical University, Zunyi 563000, Guizhou, China; Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, China.
Abstract:
This study investigates two extraction methods-ultrasound-assisted extraction (UAE) and compound enzyme extraction (CE)-for the isolation of Lonicera macranthoides polysaccharides (LMP) and evaluates their physicochemical properties, bioactivity, and therapeutic potential. The optimization of extraction conditions was performed using single-factor experiments and response surface methodology. The yield of LMP from UAE was optimized to 8.02 % ± 0.12 %, while the yield from CE was 10.03 % ± 0.32 %. Both polysaccharide extracts were characterized in terms of chemical composition, monosaccharide composition, molecular weight, thermal stability, and functional group analysis. Notably, UAE-LMP exhibited superior antioxidant, hypolipidemic, nitrite scavenging, and anti-tumor activities compared to CE-LMP. The ultrasound-assisted method enhanced the stability and bioactivity of LMP, with improved water- and oil-holding capacities and the integrity of the active groups. Our findings demonstrate that the extraction method significantly influences the structural and functional properties of LMP, suggesting that UAE may be a more effective approach for extracting bioactive polysaccharides with potential applications in medicine and nutrition.
