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Directing Proteins to the Rough Endoplasmic Reticulum01:34

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Updated: May 28, 2025

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
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Rpl12 is a conserved ribophagy receptor.

Yuting Chen1,2, Jiaxin Hu3, Pengwei Zhao1

  • 1Department of Biochemistry and Department of Hepatobiliary and Pancreatic Surgery of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.

Nature Cell Biology
|February 11, 2025
PubMed
Summary
This summary is machine-generated.

Rpl12 is identified as a conserved receptor for ribophagy, a process crucial for ribosome turnover. This finding is vital for cellular homeostasis, impacting starvation, aging, and disease models.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Autophagy Research

Background:

  • Ribophagy is a selective autophagic process regulating ribosome turnover.
  • NUFIP1, a mammalian ribophagy receptor, lacks homologues in yeast and nematodes.

Purpose of the Study:

  • To identify conserved ribophagy receptors beyond NUFIP1.
  • To elucidate the role of Rpl12 in ribophagy and cellular homeostasis across different organisms.

Main Methods:

  • Investigated Rpl12-Atg8s binding interactions.
  • Analyzed Atg1-mediated Rpl12 phosphorylation and its role in Atg11 association.
  • Utilized Caenorhabditis elegans and Drosophila melanogaster models to study ribophagy deficiency.

Main Results:

  • Rpl12 identified as a conserved ribophagy receptor.
  • Disruption of Rpl12-Atg8s binding causes ribosomal protein and rRNA accumulation.
  • Rpl12 phosphorylation enhances Atg11 binding, triggering ribophagy during starvation.
  • Ribophagy deficiency accelerates cell death, impairs development, shortens lifespan, and causes motor deficits.
  • RPL12 overexpression rescues starvation, aging, and Aβ-induced movement defects in flies.

Conclusions:

  • Rpl12 acts as a conserved ribophagy receptor essential for ribosome metabolism.
  • Ribophagy mediated by Rpl12 is critical for cellular homeostasis, organismal health, and longevity.
  • Rpl12 is a potential therapeutic target for age-related and neurodegenerative disorders.