Related Experiment Video
Updated: May 28, 2025
Characterization of Leukocyte-platelet Rich Fibrin, A Novel Biomaterial
Published on: September 29, 2015
Effects of Advanced Platelet Rich Fibrin (A-PRF+), Enamel Matrix Derivative (EMD) and Open Flap Debridement on
L Pitzurra1, D Vasdravellis1, N A M Rosema1
1Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, Netherlands.
Aim:
To study the effects of advanced platelet-rich fibrin (A-PRF+) and enamel matrix derivative (EMD) compared to open flap debridement (OFD) alone in molar furcation sites grade II on clinical and wound healing parameters.
Materials And Methods:
A randomized controlled trial was designed. Eligible patients were randomly allocated to one of three treatment groups: A-PRF+, EMD or OFD. The patients and clinical examiners were blinded for the treatment received. A minimally invasive microsurgical approach was performed for the three modalities. Clinical measurements were scored at baseline and 6 months post-operatively. The clinical healing of each furcation was scored via the Early Wound Healing Index on day 3, 1 week, 2 weeks and 6 weeks.
Results:
17 patients (A-PRF+ n = 6, EMD n = 5, OFD n = 6) completed the 6 months of follow-up. The further completion of the trial had to be cancelled due to the COVID-19 pandemic. In three patients in the A-PRF+ group, the grade II of the treated furcation regressed to grade I; the corresponding number in the EMD and OFD groups was zero and one respectively. Further, 3, 1 and 4 patients in the PRF, EMD and OFD groups respectively, showed a gain of bone level ≥1 mm. The defects in the A-PRF+ group showed delayed early healing compared to the EMD and OFD groups.
Conclusion:
The case series (RCT design) suggests a slight advantage for A-PRF+ over EMD and OFD, regarding the regressing of a furcation II to grade I; however this treatment showed delayed early wound healing compared to EMD or OFD.
Clinical Trial Registration:
https://www.isrctn.com/, identifier ISRCTN13520922.
Related Concept Videos
01:16Clot Retraction and Fibrinolysis
01:22Formation of the Platelet Plug
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
01:28Phases of Wound Repair
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...