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New nucleopolyhedrovirus isolate in the management of Spodoptera eridania
Alixelhe P Damascena1, Fernando Z Madalon2, Luis M DE Araujo Junior3
1Universidade Federal do Espírito Santo, Centro de Ciências Agrárias e Engenharias, Departamento de Agronomia, Alto Universitário, s/n, Guararema, 29500-000 Alegre, ES, Brazil.
Abstract:
Spodoptera eridania is considered a polyphagous pest, as it attacks several crops. The aim of this study was to identify a virus isolate present in symptomatic S. eridania caterpillars. The virus sample was extracted and OBs purification performed. Viral DNA was extracted using the PureLink Viral RNA/DNA Mini Kit (ThermoFisher) and primers prl8-1 and prl8-1B were used for amplification of the gene fragment. The isolate was identified showing high genetic similarity with the isolate "Spodoptera eridania nucleopolyhedrovirus isolate CNPSo-165", characterizing itself as a novelty in the scenario and as promising, adding the tactics available for the integrated management of S. eridania.
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