Unveiling the mechanism of microbial nitrogen conversion by acclimating bioanode potential for enhancing ammonia...

The biodegradation mechanism of enhancing ammonia removal and reducing N₂O emission by acclimating bioanode potential in bioelectrochemical systems (BES) was studied in this work. The BES was started up and optimized with different parameters (i.e., aeration volume, external resistance, and pH value). The cyclic voltammetry was then performed to determine the potential range for acclimating bioanode, suggesting that potentials of -0.3, -0.1, 0.1, 0.2, and 0.3 V were suitable for acclimation. The experimental results indicated that optimum total nitrogen removal was achieved at potential of 0.1 V with a removal efficiency of 83.7 %, which was 1.6 times higher than that of the control group, while the corresponding N₂O emission of 1.3 mg-N/L was detected which reduced 2.8 times. The microbial community analysis indicated that the dominant genera for enhancing nitrogen conversion were Sphaerocheata, Petrimonas, and Lentimicrobium. The electrochemical tests suggested that potential acclimation could simultaneously enhance direct electron transfer and mediated electron transfer (MET), thereby increasing extracellular electron transfer, in which the proportion of MET process mediated by riboflavin played a decisive role. Meanwhile, the intracellular electron transfer process was also strengthened by the increase of the related enzyme activity and the corresponding electron transport system activity increased 3.4 times. Additionally, nitrogen conversion pathways were proposed, indicating that potential acclimation could regulate the ammonia degradation pathway, and reduce the intermediates accumulation. These findings provide new understanding of the nitrogen conversion mechanisms in BES for ammonia removal by acclimating bioanode potential.