Interventional Effects of Edible Bird's Nest and Free Sialic Acids on LPS-Induced Brain Inflammation in Mice

Objectives: Our study investigated the effects and mechanisms of edible bird's nest (EBN) and free sialic acids (SA) on LPS-induced brain inflammation in mice. Methods: The experiment divided the mice into four groups: control group (CON), lipopolysaccharide group (LPS), EBN intervention group (EBN, 200 mg/kg/d in dry EBN), and sialic acid intervention group (SA, dosage was calibrated based on the concentration of sialic acid in EBN). Results: The results showed that LPS caused a decrease followed by upregulation in body weight in female mice, and EBN exhibited renal protective effects. In the Morris water maze, the learning and memory abilities of mice in the LPS group first declined and then recovered. At the same time, the escape latency improved in the EBN and SA groups. In the Open field test, both the EBN and SA groups exhibited anti-anxiety and anti-depressive effects. Immunohistochemistry in the hippocampus showed significant cell damage in the LPS group, while the damage was alleviated in the EBN and SA groups. LPS promoted the expression of TICAM1 and MYD88 in the NF-κB pathway, while both the EBN and SA groups could inhibit the expression of TICAM1. Conclusions: The study has found that both EBN and SA exhibited noteworthy anti-inflammatory effects, indicating that the main active component in EBN that provides neuroprotective effects is SA. The bound SA in EBN confers additional effects, supporting the development of prevention and treatment strategies for brain inflammation.