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Double Peptide-Functionalized Carboxymethyl Chitosan-Coated Liposomes Loaded with Dexamethasone as a Potential
Loredana Iftode1,2, Anca Niculina Cadinoiu3, Delia Mihaela Raţă3
1Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania.
International Journal of Molecular Sciences
|February 13, 2025
Summary
New cationic liposomes coated with dual-peptide functionalized carboxymethyl chitosan (CMCS) show promise for inner ear disease treatment. These targeted nanocarriers demonstrate hemocompatibility and minimal cytotoxicity, enhancing drug delivery potential.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Drug Delivery
Background:
- Liposomes are biocompatible nanocarriers widely used in medicine.
- Functionalizing liposomes with specific ligands enhances cellular uptake and therapeutic efficacy.
- Inner ear diseases require targeted drug delivery systems for effective treatment.
Purpose of the Study:
- To develop novel cationic liposomes coated with dual-peptide functionalized carboxymethyl chitosan (CMCS) for inner ear disease treatment.
- To functionalize CMCS with Tet1 and TAT peptides for targeted delivery and cellular penetration.
- To load dexamethasone phosphate as a model anti-inflammatory drug.
Main Methods:
- Carboxymethyl chitosan (CMCS) was functionalized with Tet1 and TAT peptides.
- Cationic liposomes were prepared and coated with the functionalized CMCS.
- Dexamethasone phosphate was loaded into the liposomes.
- Characterization included infrared spectroscopy, transmission electron microscopy (TEM), and drug release studies.
- Hemocompatibility and cytotoxicity assays were performed.
Main Results:
- Infrared spectroscopy confirmed successful CMCS functionalization with peptides.
- Liposome size ranged from 167-202 nm, with a spherical, unilamellar structure observed via TEM.
- CMCS-coated liposomes modulated dexamethasone phosphate release compared to uncoated liposomes.
- Liposomes exhibited hemocompatibility with <2.5% erythrocyte lysis at 100 µg/mL.
- Peptide-functionalized CMCS-coated liposomes showed minimal cytotoxicity to V79-4 cells.
Conclusions:
- Dual-peptide functionalized CMCS-coated liposomes are a promising nanocarrier system for inner ear disease treatment.
- The developed liposomes demonstrate good biocompatibility, hemocompatibility, and targeted delivery potential.
- These findings support the advancement of peptide-functionalized liposomes for enhanced therapeutic efficacy in otological applications.
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