Corydalis Tuber Extract Alleviates Atopic Dermatitis: Transcriptomics-Based Mechanism Prediction and In Vitro/In Vivo...

Area of Science:

• Dermatology
• Immunology
• Pharmacology

Background:

• Atopic dermatitis (AD) is a prevalent inflammatory skin condition causing eczema and chronic itching.
• Current treatments may have limitations, necessitating exploration of novel therapeutic agents.

Purpose of the Study:

• To investigate the therapeutic effects and underlying mechanisms of Corydalis Tuber extract (CTE) on atopic dermatitis.
• To evaluate CTE's impact on inflammatory responses in both cellular models and an animal model of AD.

Main Methods:

• Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) identified compounds in CTE.
• Transcriptome analysis elucidated CTE's mechanism on tumor necrosis factor-α- and interferon-γ-stimulated human keratinocytes (HaCaT cells).
• A house dust mite-induced mouse model assessed CTE's topical efficacy and its effects on various biomarkers.

Main Results:

• CTE downregulated cytokine signaling and chemokine receptor pathways in HaCaT cells, inhibiting CCL5, CCL17, and CCL22 via JAK/STAT and NF-κB pathways.
• In AD mice, topical CTE reduced dermatitis scores, epidermal thickness, and inflammatory cell infiltration.
• CTE decreased plasma levels of histamine, IgE, CCL17, CCL22, corticosterone, and cortisol, and reduced thymic stromal lymphopoietin, CD4+ T cells, IL-4, and ICAM-1 in skin lesions.

Conclusions:

• Corydalis Tuber extract demonstrates significant anti-atopic dermatitis effects.
• CTE alleviates AD by modulating inflammatory mediators, cytokines, chemokines, and stress-related factors, offering a potential natural therapeutic option.