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Related Experiment Videos

Oxygen dependent lactate utilization by Lactobacillus plantarum.

M G Murphy, L O'Connor, D Walsh

    Archives of Microbiology
    |February 1, 1985
    PubMed
    Summary

    Lactobacillus plantarum P5 utilizes lactate for aerobic growth, converting it to acetate. This study proposes and validates the metabolic pathway involved in this oxygen-dependent energy generation.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Metabolic pathways

    Background:

    • Lactate metabolism is crucial for various microorganisms.
    • Understanding aerobic respiration in lactic acid bacteria provides insights into energy production.

    Purpose of the Study:

    • To investigate the aerobic metabolism of lactate by Lactobacillus plantarum P5.
    • To elucidate the specific metabolic pathway and enzymes involved in lactate conversion to acetate.

    Main Methods:

    • Culturing Lactobacillus plantarum P5 under aerobic conditions with lactate.
    • Analyzing growth media for lactate consumption and acetate production.
    • Enzyme assays using cell-free extracts to demonstrate key enzyme activities.
    • Characterization of intermediates like pyruvate and acetyl phosphate.

    Main Results:

    • Lactobacillus plantarum P5 exhibits aerobic growth dependent on lactate.
    • Lactate is converted to acetate, with acetate accumulating in the medium.
    • Enzymes including lactic dehydrogenases, pyruvate oxidase, acetate kinase, and NADH oxidase were identified and demonstrated.
    • The proposed metabolic pathway involving pyruvate and acetyl phosphate was supported by experimental evidence.

    Conclusions:

    • Lactobacillus plantarum P5 employs an oxygen-dependent pathway for aerobic growth using lactate.
    • This pathway generates adenosine triphosphate (ATP) through the conversion of lactate to acetate.
    • The study identifies the key enzymes and intermediates in this significant metabolic process.

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