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Curcumin Analog GO-Y030 Triggers JNK and p38 Signalling to Activate Apoptotic Cascades in Human Osteosarcoma Cells

Yu-Hsien Lin1,2, Jia-Sin Yang1,3, Chia-Hsuan Chou1,3

  • 1Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.

Journal of Cellular and Molecular Medicine
|February 13, 2025
PubMed

Insights

A novel curcumin analog, GO-Y030, effectively induces apoptosis in human osteosarcoma cells by activating key cell death pathways. This research offers new therapeutic strategies for osteosarcoma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Osteosarcoma is a primary bone cancer with high metastatic potential, particularly in adolescents.
  • Curcumin's low bioavailability limits its effectiveness as an adjuvant therapy for osteosarcoma.
  • There is a need for novel therapeutic agents to improve outcomes for osteosarcoma patients.

Purpose of the Study:

  • To investigate the apoptosis-inducing effects of the synthesized curcumin analog GO-Y030 in human osteosarcoma cells.
  • To elucidate the molecular mechanisms underlying GO-Y030's action against osteosarcoma.
  • To assess GO-Y030's potential as a therapeutic agent for osteosarcoma.

Main Methods:

  • Flow cytometry and Annexin V/propidium iodide staining were used to assess apoptosis.
  • Human apoptosis arrays and Western blotting were employed to analyze protein expression and activation.
  • Specific pathway inhibitors (JNK, p38, ERK) were utilized to determine the role of signaling pathways.

Main Results:

  • GO-Y030 dose-dependently reduced osteosarcoma cell viability and induced apoptosis.
  • GO-Y030 activated caspases 8, 9, and 3, and modulated inhibitor of apoptosis proteins (IAPs).
  • GO-Y030 increased phosphorylation of JNK1/2 and p38, which were crucial for GO-Y030-induced apoptosis, while ERK activation was not essential.

Conclusions:

  • GO-Y030 effectively triggers both extrinsic and intrinsic apoptotic pathways in human osteosarcoma cells.
  • The JNK1/2 and p38 signaling pathways are critical mediators of GO-Y030's anti-osteosarcoma activity.
  • GO-Y030 demonstrates significant potential as a therapeutic agent for osteosarcoma.
Keywords:
GO‐Y030JNKapoptosiscurcuminosteosarcomap38

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