Acetate prevents pistil dysfunction in rice under heat stress by inducing methyl jasmonate and quercetin synthesis

Acetic acid (HAC) is a crucial signal molecule in plant stress responses; however, its role in conferring heat tolerance to rice remains unclear.

This study aims to investigate the effect of HAC in protecting pistil function under heat stress and its potential role in facilitating pollen germination and tube growth via HAC-induced synthesis of methyl jasmonate (MeJA) and quercetin (QR).

Physiological analysis, including pollen germination, pollen tube growth into the ovule, reactive oxygen species (ROS), as well as the levels of HAC, acetyl coenzyme A (acetyl-CoA), MeJA, and QR in the pistils of heat stress-treated early indica rice cultivars Zhongzao39 (ZZ39) and Zhongjiazao17 (ZJZ17), were conducted. RNA sequencing (RNA-seq) was performed to identify differentially expressed genes involved in this process. Effect of exogenous acetate (NaAC), MeJA, and QR on spikelet fertility were also investigated.

Compared with ZJZ17, severe inhibition of spikelet fertility, pollen germination, and pollen tube growth was observed in ZZ39, due to the ROS burst and an irregular distribution across the stigma, style, and ovule. RNA-seq and physiological data indicate that HAC may activate acetyl-CoA to enhance heat tolerance by inducing the synthesis of MeJA and QR. Exogenous NaAC enhanced spikelet fertility under heat stress, accompanied by elevated antioxidant enzyme activities, improved energy status, and increased levels of acetyl-CoA, MeJA, and QR in the pistils. Additionally, NaAC, MeJA, and QR, either alone or in combination, effectively augmented spikelet fertility under heat stress, while the combination of MeJA and QR inhibitors significantly reduced fertility.

Acetate activates acetyl-CoA to induce the synthesis of both MeJA and QR, thereby alleviating heat-induced pistil dysfunction by maintaining ROS homeostasis and enhancing the pollen germination, pollen tube growth and spikelet fertility. Our results offer a promising strategy to enhance the heat tolerance of crops.