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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
Published on: August 16, 2024
Unveiling a novel RNA G-triplex structure: its function and potential in CRISPR-based diagnostics
Tao Li1,2, Runchen Li3,4, Zheyu Li1,2
1School of Laboratory Medicine, Hubei University of Chinese Medicine, 16 Huangjia Lake West Road, Wuhan 430065, China. yingli@hbucm.edu.cn.
Abstract:
We report the discovery of a novel higher-order RNA structure, RNA G-triplex (rG3), formed by the TERRA sequence. Through CD spectroscopy, NMR analysis, and molecular modeling, we confirmed its stable, parallel conformation. rG3 exhibits strong binding to thioflavin T (ThT), N-methyl mesoporphyrin IX (NMM), and hemin, showcasing its potential as a biosensing element. Additionally, CRISPR-Cas13a trans-cleaves rG3, demonstrating its utility as a sensitive reporter in diagnostic applications. These findings expand the structural diversity of RNA and suggest new avenues for RNA-based biosensors and CRISPR diagnostics.
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