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Updated: Aug 5, 2026

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
Published on: September 3, 2014
Membrane proteins and glycoproteins specific to central nervous system axons and growth cones
Abstract:
The specific functions of axons with growth cones might be correlated with a specific molecular composition differing from that of the perikarya. Thus axons with growth cones and cell perikaryal fractions were isolated by microdissection from neural retina explants of 6-day-old chick embryos. The protein composition of the minute amount of material available was analyzed with a two-dimensional micro-gel electrophoresis system in combination with various labeling procedures for surface- and glycoproteins. All methods showed, besides common features, proteins specific for axons with growth cones and for cell bodies.
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Neurons: The Axon
The axon attaches to the cell body at a cone-shaped elevation called the axon hillock. The initial part of the axon, closest to the hillock, is known as the initial segment.

