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Published on: May 4, 2018
LINC00673 binds with leptin to regulate osteogenic function in periodontal ligament stem cells
Yang Yang1,2, Peng Yang1, Yuxing Zhang1
1Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University &Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, No.44-1 Wenhua Road West, Shandong, 250012, Jinan, China.
A regulatory network involving LINC00673 and microRNA-188-3p influences bone formation in periodontal ligament stem cells. This interaction impacts leptin expression, offering potential therapeutic targets for diabetes-related periodontitis.
Area of Science:
- Molecular Biology
- Stem Cell Biology
- Periodontology
Background:
- Advanced glycation end products (AGEs) create a microenvironment impacting periodontal ligament stem cells (PDLSCs).
- The roles of LINC00673 and leptin in AGEs-induced PDLSC osteogenesis require investigation.
Purpose of the Study:
- To investigate the association between LINC00673, leptin, and PDLSC osteogenesis under AGEs.
- To elucidate the regulatory network involving LINC00673, miR-188-3p, and leptin in PDLSC osteogenic differentiation.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) for LINC00673 expression during osteogenic differentiation.
- Alkaline phosphatase and Alizarin red S staining to assess osteogenesis.
- Nude mouse dorsal assays for bone regeneration and Western blot for osteoblast markers.
Main Results:
- MiR-188-3p directly interacts with LINC00673, promoting osteogenic differentiation.
- LINC00673 acts as a competing endogenous RNA for miR-188-3p, reversing the pro-osteogenic effect.
- A LINC00673/miR-188-3p network regulates leptin (LEP) expression, inhibiting the Wnt pathway and reducing bone formation.
Conclusions:
- A regulatory network between long non-coding RNA (lncRNA) LINC00673 and microRNA (miRNA) miR-188-3p governs PDLSC osteogenic differentiation.
- This lncRNA-miRNA network regulates leptin, affecting bone formation.
- The identified regulatory network presents a potential therapeutic target for diabetes-related periodontitis.
