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Dual-functions of prime-and-rinse approach with MDP primer on the performance of modified self-etch adhesive
Bing Liang1, Xiaolu Chen1, Yi Sun1
1Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province, Hangzhou 310000, PR China.
Objectives:
This study introduced the prime-and-rinse approach with an methacryloyloxydecyl dihydrogen phosphate (MDP)-containing primer to augment the performance of modified self-etch adhesives loaded with amorphous fluorinated calcium phosphate (AFCP) nanoparticles.
Methods:
The modified self-etch adhesive was acquired by adding 20 wt% AFCP nanoparticles into Clearfil S3 Bond. An experimental primer containing 15 wt% MDP was prepared. The bond performance was assessed through micro-tensile bond strength (MTBS) tests. Additionally, a single-layer collagen model and demineralized dentin were used to investigate mineralization characteristics. Cytotoxicity was evaluated using a modified transwell insert model on human dental pulp stem cells.
Results:
The incorporation of AFCP nanoparticles into the Clearfil S3 Bond led to a significant reduction in MTBS. While the application of MDP primer could improve the bond performance of modified Clearfil S3 Bond. The MDP primer further augmented the mineralization of single-layer collagen model and demineralized dentin induced by modified self-etched adhesive. The relative cell viability was around 100 % in all groups in the study.
Clinical Significance:
These results indicated that the prime-and-rinse approach with MDP primer not only compensated for the reduced bond strength caused by the incorporation of AFCP nanoparticles but also enhanced mineralization capabilities and maintained biocompatibility, offering a promising strategy for improving the longevity of dental restorations.
