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Long non-coding RNA NEAT1 promotes colorectal cancer progression via interacting with SIRT1
Yuwei Li1, Yunchun Xu1, Xinya Yu1
1Department of Medical Microbiology and Immunology, School of Basic Medical Sciences, Dali University, Dali, Yunnan, P.R. China.
Scientific Reports
|February 15, 2025
Summary
Nuclear-enriched abundant transcript 1 (NEAT1) promotes colorectal cancer (CRC) progression and metastasis. Reduced NEAT1 levels inhibit CRC cell proliferation and invasion, suggesting NEAT1 as a potential prognostic biomarker and therapeutic target for CRC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Nuclear-enriched abundant transcript 1 (NEAT1) is a long noncoding RNA implicated in various cancers.
- Its specific role and regulatory mechanisms in colorectal cancer (CRC) progression remain incompletely understood.
Purpose of the Study:
- To investigate the expression, clinical significance, and functional role of NEAT1 in colorectal cancer.
- To elucidate the underlying molecular mechanisms, including downstream targets and immune cell infiltration, by which NEAT1 influences CRC progression.
Main Methods:
- Analysis of NEAT1 expression in The Cancer Genome Atlas (TCGA) database and 50 CRC specimens.
- Gene Set Enrichment Analysis (GSEA), CancerSEA, and immune infiltration studies.
- In vitro assays including CCK8, wound healing, and Transwell assays; immunohistochemistry for downstream target validation.
Main Results:
- Increased NEAT1 expression correlates with adverse outcomes in colorectal tissues.
- Reduced NEAT1 levels significantly inhibit CRC cell proliferation, invasion, and migration in vitro.
- NEAT1 promotes CRC progression and metastasis via upregulation of Sirtuin 1 (SIRT1) and influences immune cell infiltration.
Conclusions:
- NEAT1 acts as an oncogene in colorectal cancer, promoting tumor progression and metastasis.
- NEAT1's mechanism involves the upregulation of SIRT1 and impacts immune cell infiltration.
- NEAT1 shows potential as a prognostic biomarker and therapeutic target for colorectal cancer.
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