Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene mapping by chromosome spot hybridization.

J G Collard, P A de Boer, J W Janssen

    Cytometry
    |May 1, 1985
    PubMed
    Summary

    This study introduces a rapid method for gene localization using flow-sorted chromosomes and DNA probes. The technique successfully mapped the human c-myb oncogene to chromosome 6, demonstrating its efficiency for gene assignment.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Straylight and Pupil Size in Cataract Patients.

    Current eye research·2025
    Same author

    Modeling the Transport of Human Rotavirus and Norovirus in Standardized and in Natural Soil Matrix-Water Systems.

    Food and environmental virology·2019
    Same author

    Suicide after inhaling a pyrethrins containing insecticide spray.

    BMJ case reports·2019
    Same author

    Evaluation of Bacterial Contamination as an Indicator of Viral Contamination in a Sedimentary Aquifer in Uruguay.

    Food and environmental virology·2018
    Same author

    Emerging Salmonella Enteritidis anaerogenic phage type 14b: Outbreak in Norwegian, Swedish and Finnish travellers returning from Greece.

    Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2017
    Same author

    Multi-country outbreak of Salmonella enteritidis infection linked to the international ice hockey tournament.

    Epidemiology and infection·2017

    Area of Science:

    • Genetics
    • Molecular Biology
    • Cytogenetics

    Background:

    • Gene localization is crucial for understanding genetic diseases and functions.
    • Existing methods for gene mapping can be time-consuming and complex.

    Purpose of the Study:

    • To develop a rapid and simple method for localizing cloned single-copy genes to specific chromosomes.
    • To demonstrate the utility of the method for sublocalizing genes using translocated chromosomes.

    Main Methods:

    • Flow-sorting of chromosomes directly onto nitrocellulose filters.
    • Hybridization of chromosomal DNA with gene-specific radioactively labeled DNA probes.
    • Utilizing mild aspiration to concentrate sorted chromosomes for enhanced detection.

    Main Results:

    • The technique successfully localized the human c-myb oncogene to the 6q21-q23 region by sorting translocated chromosomes.
    • Sorting 10,000-30,000 chromosomes was sufficient for detecting single-copy gene hybridization.
    • The method proved rapid and simple for assigning cloned genes to chromosomes.

    Conclusions:

    • Chromosome spot hybridization is an effective and efficient technique for gene mapping.
    • This method facilitates the rapid assignment and sublocalization of genes.
    • It offers a valuable tool for genetic research and diagnostics.

    Related Experiment Videos