Advancing extrusion-based embedded 3D bioprinting via scientific, engineering, and process innovations

Area of Science:

• Biotechnology
• Regenerative Medicine
• Materials Science

Background:

• Extrusion-based embedded 3D bioprinting utilizes a support bath for fabricating complex biologic constructs.
• This advanced technique has significantly broadened the scope of achievable tissue architectures.

Purpose of the Study:

• To explore future advancements in extrusion-based embedded 3D bioprinting.
• To address existing scientific, engineering, and process-related challenges.
• To facilitate the clinical translation of bioprinted tissues.

Main Methods:

• Perspective review of current challenges and future directions in extrusion-based embedded 3D bioprinting.
• Analysis of scientific, engineering, and process-related limitations.
• Identification of potential solutions and innovations.

Main Results:

• Significant challenges persist in achieving full anatomic structure and physiologic function for therapeutic applications.
• Future advances are crucial for overcoming limitations in resolution, material properties, and cell viability.
• Technological innovations are expected to enhance the precision and scalability of the bioprinting process.

Conclusions:

• Overcoming current challenges in extrusion-based embedded 3D bioprinting is essential for its clinical application.
• Continued research and development in bioink formulation, printing technology, and post-printing maturation are critical.
• Successful translation of bioprinted tissues holds immense promise for regenerative medicine and therapeutic interventions.