Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correlation analysis of arterial blood lactate levels with major in-hospital adverse cardiovascular events after coronary intervention in patients with acute myocardial infarction.

BMC research notes·2026
Same author

Akkermansia muciniphila-derived postbiotics reprogram immune balance to combat sepsis via the IDO1/Kyn/AhR metabolic axis.

Journal of advanced research·2026
Same author

Targeting TARDBP to Restore Colonic Barrier Integrity in Ulcerative Colitis via NFATC1 mRNA Destabilization.

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology·2026
Same author

Valorization of lithium slag into non-sintered lightweight aggregates: Long-term immobilization of Be/Tl and environmental safety evaluation.

Journal of hazardous materials·2026
Same author

14-3-3η protein ameliorates glomerular endothelial cell injury in lupus nephritis by regulating mTOR pathway-mediated autophagy.

European journal of pharmacology·2026
Same author

A Conformer-Based Time-Frequency Decoupling Network for Pig Vocalization Behavior Classification.

Animals : an open access journal from MDPI·2026

Related Experiment Video

Updated: May 27, 2025

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

10.6K

Transcriptome analysis of 3D4/21 cells expressing CSFV NS4B.

Wang Dong1, Huifang Lv1, Yuzhen Song1

  • 1Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine and Pharmacy, Henan University of Animal Husbandry and Economy, Zhengzhou, China.

Frontiers in Microbiology
|February 19, 2025
PubMed
Summary

Classical swine fever virus (CSFV) non-structural protein 4B (NS4B) impacts host cell processes. This study reveals NS4B regulates endocytosis, autophagy, and immune responses, offering insights into CSFV pathogenesis.

Keywords:
NS4BRNA-seqautophagyclassical swine fever virusendocytosis

More Related Videos

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

15.4K
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
09:13

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

17.3K

Related Experiment Videos

Last Updated: May 27, 2025

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
13:13

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

10.6K
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
09:26

Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR

Published on: January 22, 2014

15.4K
Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
09:13

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

17.3K

Area of Science:

  • * Virology
  • * Molecular Biology
  • * Cell Biology

Background:

  • * Classical swine fever (CSF) causes significant global economic losses in the pig industry.
  • * Classical swine fever virus (CSFV) non-structural protein 4B (NS4B) is vital for viral replication and pathogenicity.
  • * The precise functions of CSFV NS4B during infection remain incompletely understood.

Purpose of the Study:

  • * To investigate the molecular mechanisms and cellular functions of CSFV NS4B.
  • * To identify host genes differentially expressed in response to CSFV NS4B expression using RNA-sequencing.
  • * To elucidate the role of NS4B in host cell processes such as endocytosis, autophagy, and immune response.

Main Methods:

  • * RNA-sequencing (RNA-seq) was employed to profile gene expression in 3D4/21 cells expressing CSFV NS4B.
  • * Differentially expressed genes (DEGs) were identified by comparing NS4B-expressing cells to control cells (empty vector).
  • * Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. RT-qPCR was used for validation of selected DEGs.

Main Results:

  • * A total of 4397 DEGs were identified in cells expressing CSFV NS4B.
  • * Enrichment analyses indicated involvement of DEGs in endocytosis, autophagy, cell adhesion, transport, immune response, and apoptosis.
  • * Key findings include regulation of endocytosis-related genes, downregulation of antiviral immune genes (e.g., RIG-I, MDA5), and upregulation of autophagy-related genes (e.g., WIPI2, ATG16L2).

Conclusions:

  • * CSFV NS4B plays a significant role in modulating host cellular processes crucial for viral invasion and intracellular trafficking.
  • * NS4B actively induces host autophagy and suppresses the host's antiviral immune response.
  • * This research provides valuable insights into the multifaceted functions of CSFV NS4B during infection, aiding in understanding viral pathogenesis.