Related Experiment Video
Updated: May 27, 2025
11:48
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
Published on: July 24, 2013
15.8K
[Adapting Mouse Genome Editing Technique from Scratch Using in utero Electroporation]
J V Popova1,2, V D Bets3, E S Omelina1
1Institute of Molecular and Cellular Biology, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia.
Molekuliarnaia Biologiia
|February 19, 2025
Summary
This study presents a cost-effective mouse genome editing pipeline using improved Genome-editing via Oviductal Nucleic Acids Delivery (i-GONAD) and CRISPR/Cas9. The method simplifies generating genetically modified mice, requiring less expertise and equipment.
Area of Science:
- * Molecular Biology
- * Genetics
- * Bioengineering
Context:
- * Traditional mouse genome modification is expensive and requires specialized skills.
- * Zygote electroporation techniques offer high gene delivery efficiency.
- * The i-GONAD method enables in utero electroporation for gene transfer.
Purpose:
- * To develop an accessible and economical mouse genome-editing pipeline.
- * To adapt the i-GONAD technology for CRISPR/Cas9-mediated gene editing.
- * To establish a simplified protocol for generating knockout mice with minimal resources.
Summary:
- * The study successfully employed CRISPR/Cas9 with i-GONAD for mouse genome editing.
- * A pipeline was developed for generating Il10 knockout C57BL/6 mice.
- * The approach demonstrated feasibility with 23% of pups showing genetic modification at the Il10 locus.
Impact:
- * Provides a cost-effective and user-friendly method for in-house mouse transgenesis.
- * Reduces the need for extensive equipment and operator expertise.
- * Facilitates broader access to genome editing technologies for researchers.
