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Cooperation of R and Rab5 regulates crayfish anti-disease immune response by triggering apoptosis

Xin Ren1, Bo Peng1, Yunfei Tan1

  • 1National Key Laboratory of Crop Genetic Improvement, Shuangshui Shuanglü Institute, Huazhong Agricultural University, Wuhan 430070, China; College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.

International Journal of Biological Macromolecules
|February 19, 2025
PubMed

Insights

Crayfish immunity involves Toll-like receptors (TLRs). This study reveals the resistance (R) protein interacts with MyD88 and Rab5, suggesting exosomes play a role in TLR-mediated immune responses in crayfish.

Area of Science:

  • Aquatic immunology
  • Invertebrate innate immunity
  • Molecular biology

Background:

  • Toll-like receptors (TLRs) are crucial for innate immunity in aquatic species.
  • The specific regulatory mechanisms of TLRs in crayfish (Procambarus clarkii) immunity remain largely unknown.
  • Previous research identified a resistance (R)-gene in crayfish.

Purpose of the Study:

  • To investigate the regulatory pathways of Toll-like receptors (TLRs) in crayfish.
  • To identify proteins interacting with TLRs, specifically those encoded by the R-gene.
  • To explore the potential role of exosomes in TLR-mediated immune responses.

Main Methods:

  • In vivo and in vitro pull-down assays to identify protein interactions.
  • Transcriptome analysis of RNA interference (RNAi) targeting Rab5 and GFP in crayfish hemocytes.
  • Western blotting to validate protein expression of exosome-related genes.

Main Results:

  • The R protein interacts with myeloid differentiation factor 88 (MyD88) and Ras-like small GTPase Rab5.
  • Direct interaction was confirmed between R and MyD88, but not R and Rab5.
  • Differentially expressed genes in the Toll signaling pathway were identified, along with exosome-related genes (TSG101, CD9).

Conclusions:

  • A hypothetical model suggests the R protein, MyD88, and Rab5-cooperating exosomes mediate immune responses and apoptosis upon pathogen challenge.
  • This study provides preliminary evidence for exosome involvement in TLR-mediated immune regulation in crayfish.
  • Findings advance the understanding of immune resistance pathways in crayfish.
Keywords:
ApoptosisImmune regulationInteracting proteinsR proteinRed swamp crayfish (Procambarus clarkii)

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