Related Experiment Video
Updated: May 27, 2025

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
Published on: March 20, 2016
TEOSINTE BRANCHED1/CYCLOIDEA/PCF protein PeTCP4s positively regulate lateral bud development by activating PePIN2a
Xin Chang1, Jiaoyu Chen1, Yujiao Liu1
1State Key Laboratory of Subtropical Silviculture, Bamboo Industry Institute, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China.
Abstract:
The development of lateral buds on the underground rhizome in bamboo is a major determinant of the yield of bamboo shoots. However, the regulating factors influencing this developmental process and the molecular mechanisms remain largely unknown. Here, we found that treatment with the Cytokinin significantly increased the lateral bud outgrowth of Moso bamboo (Phyllostachys edulis). A pair of plant-specific TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) genes, PeTCP4a and PeTCP4b, were identified in Moso bamboo. The expression of both PeTCP4 genes was promoted by Cytokinin and synchronized with the development of lateral buds. Overexpression of PeTCP4 genes exhibited increased rosette-leaf branches. PeTCP4s upregulated the expression of auxin efflux carrier PIN-FORMED (PIN2) gene and those genes involved in lateral organ such as KNAT2, KNAT6, STM and IAA3 in transgenic plants. Similar to PeTCP4 genes, the expression of PePIN2a in Moso bamboo was also promoted by Cytokinin. Subsequently, we found both PeTCP4 proteins directly bound to the PePIN2a gene and activated its expression. Our data suggested that PeTCP4s, induced by Cytokinin, promote lateral bud outgrowth by activating PePIN2a expression and upregulating the expression of those genes involved in lateral organ. This study may provide new insights into the mechanism of lateral bud development of bamboo.
Related Concept Videos
Cell Specific Gene Expression
C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...
Cell Signaling in Plants
Primary and Secondary Growth in Roots and Shoots
Pinching-off of Coated Vesicles
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...

