Functional Integrated Platform Initiates Cascade Reactions for Tooth-Extraction Wound Healing and Alveolar Bone...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Oral and Maxillofacial Surgery

Background:

• Alveolar bone regeneration and wound healing post-tooth extraction are crucial for maxillofacial esthetics and health.
• Developing clinically translatable scaffolds for tooth extraction management remains a significant challenge.

Purpose of the Study:

• To develop a versatile, fibrin-based platform for enhanced wound healing and bone regeneration in tooth extraction sockets.
• To investigate the platform's ability to manage infection, promote angiogenesis, and stimulate ossification.

Main Methods:

• Fabrication of a fibrin-based platform incorporating calcium phosphate nanoparticles with glucose oxidase (GOx), l-arginine, and metronidazole.
• In situ gelation in extraction sockets for hemostasis.
• Utilizing a cascade reaction involving GOx, H2O2, nitric oxide, and acidic microenvironment for controlled release and therapeutic effects.

Main Results:

• The platform demonstrated effective hemostasis upon implantation.
• In vitro and in vivo studies confirmed potent antibacterial activity and promotion of angiogenesis and ossification.
• The cascade reaction effectively combatted bacterial infection and facilitated bone regeneration.

Conclusions:

• The developed fibrin-based platform shows significant potential as a comprehensive therapeutic strategy for managing tooth extraction sockets.
• The platform effectively addresses critical aspects of healing, including hemostasis, infection control, and bone regeneration.