Structural differences in the outer membrane-associated flagellar rings between sheathed and unsheathed flagella

The bacterial flagellar motor generates a torque to move the bacterium in its environment. Despite sharing a conserved core, flagellar motors of different species exhibit structural diversity with species-specific embellishments. These embellishments are classified into various types, including integrated (spanning the whole periplasmic space) or outer membrane (OM)-associated ones. Here, we used cryo-electron tomography to investigate the structural differences between the embellishments of sheathed and unsheathed flagella in various species. We discovered that the integrated embellishments of sheathed flagella have disks and rings with a constant diameter, while those of unsheathed flagella have components that vary significantly in diameter. Both unsheathed and sheathed flagella with OM-associated embellishments have components with constant diameter with a subset of motors having an additional extracellular ring. In this Hypothesis article, we propose that these differences may play a role in the formation of the sheath, as having large protein disks of various diameters underneath the OM may interfere with membrane bending to form the sheath.