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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Updated: May 27, 2025

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl- Channels and Transporters

Published on: April 20, 2015

Lipid probes to study ion channels.

Helene Jahn¹, Show-Ling Shyng², Carsten Schultz²

¹Department of Chemical Physiology and Biochemistry, School of Medicine, Oregon Health and Science University, Portland, OR, 97239, USA; Department of Molecular Microbiology and Immunology, School of Medicine, Oregon Health and Science University, Portland, OR, 97239, USA.

Current Opinion in Chemical Biology

|February 20, 2025

Summary

Lipids regulate transmembrane proteins, including ion channels, through direct binding or by altering the surrounding membrane environment. This review explores these lipid-protein interactions and discusses methods to study them.

Keywords:

Caged lipids Interactome Ligand Photo-crosslinkable lipids Protein structure

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Area of Science:

Biochemistry
Molecular Biology
Membrane Biophysics

Background:

Lipids are crucial small molecules that interact with proteins, particularly transmembrane proteins.
Transmembrane proteins, like ion channels, are influenced by lipids via direct interactions or by modulating membrane properties.

Purpose of the Study:

To review the diverse roles of lipids in regulating ion channel function.
To provide examples of direct and indirect lipid-ion channel interactions.
To discuss current and future experimental tools for studying these interactions.

Main Methods:

Literature review of lipid-protein interactions.
Analysis of mechanisms of lipid regulation on ion channels.
Discussion of molecular tools and experimental approaches.

Main Results:

Lipids can directly bind to ion channels as ligands.
Lipids indirectly affect ion channels by altering membrane biophysical properties.
Lipids can influence protein-protein interactions and effector localization.

Conclusions:

Lipid interactions are essential for the proper function of ion channels.
Understanding lipid regulation provides insights into channelopathies.
Advanced molecular tools are needed to further elucidate lipid-ion channel dynamics.