Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a virus that...
Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Label-Free All-Electrical Tracking of Individual and Collective Cell Migration on a Megapixel CMOS Capacitance Sensor.

bioRxiv : the preprint server for biology·2026
Same author

Effect of modeling subject-specific cortical folds on brain injury risk prediction under blunt impact loading.

Biomechanics and modeling in mechanobiology·2026
Same author

Traumatic brain injury-on-a-chip: a microfluidic device for the compression of cortical spheroids.

Lab on a chip·2026
Same author

Digital Volume Correlation Challenge 2.0: A Comprehensive Dataset for Digital Volume Correlation Benchmarking.

Research square·2026
Same author

Partial EMT Drives Persistent Collective Migration via Collision Guidance in Heterogeneous Populations.

bioRxiv : the preprint server for biology·2026
Same author

Aging changes cell mechanics and dynamics associated with cytoplasmic crowding.

PNAS nexus·2026

Related Experiment Video

Updated: Jun 18, 2026

Three-Dimensional 3D Tumor Spheroid Invasion Assay
12:19

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Published on: May 1, 2015

57.2K

Collective Transitions from Orbiting to Matrix Invasion in 3D Multicellular Spheroids.

Jiwon Kim1, Hyuntae Jeong1, Carles Falcó2

  • 1School of Engineering, Legoretta Cancer Center. Brown University. 184 Hope St Box D, Providence RI 02912, USA.

Biorxiv : the Preprint Server for Biology
|February 24, 2025
PubMed
Summary
This summary is machine-generated.

Epithelial cells orbiting a curved matrix transition to radial invasion, driven by cell-matrix interactions. This collective cell behavior is key to tissue sculpting and has implications for development and disease.

More Related Videos

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
08:15

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

Published on: May 7, 2019

15.5K
Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion
09:18

Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion

Published on: April 4, 2025

419

Related Experiment Videos

Last Updated: Jun 18, 2026

Three-Dimensional 3D Tumor Spheroid Invasion Assay
12:19

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Published on: May 1, 2015

57.2K
Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay
08:15

Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay

Published on: May 7, 2019

15.5K
Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion
09:18

Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion

Published on: April 4, 2025

419

Area of Science:

  • Cell Biology
  • Biophysics
  • Developmental Biology

Background:

  • Epithelial tissues sculpt into spherical shapes through coordinated cell rotation along curved matrix interfaces.
  • Radial invasion by multicellular strands follows, involving local matrix remodeling.
  • The dynamic interplay between cells and matrix drives these symmetry-breaking transitions, but the mechanisms are not fully understood.

Purpose of the Study:

  • To investigate how epithelial cell spheroids transition from circumferential orbiting to radial invasion.
  • To elucidate the role of bidirectional cell-matrix interactions and matrix curvature in tissue morphogenesis.
  • To model the collective behaviors governing cell-matrix interactions during tissue sculpting.

Main Methods:

  • Observation of epithelial cell spheroids on curved matrix interfaces.
  • Perturbation of collective migration using osmotic pressure.
  • Development of a minimal self-propelled particle model to simulate cell-matrix interactions.

Main Results:

  • Spheroids transition from ellipsoidal to spherical shapes during orbiting.
  • Accumulation of contractile tractions at discrete sites reorients cells from orbiting to radial invasion.
  • Initial ellipsoid morphology predicts the number and orientation of invading strands.
  • Osmotic pressure can arrest orbiting and reverse invasion.
  • Mosaic spheroids with leader cells show impaired orbiting but retained invasion.

Conclusions:

  • Tissue morphogenesis is governed by collective cell behaviors and local matrix curvature.
  • Bidirectional cell-matrix interactions are crucial for transitions between orbiting and invasion.
  • The findings offer insights into embryonic development and tumor progression.