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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
Published on: December 23, 2022
A novel biosensor for highly sensitive DNA damage detection using TdT and CRISPR-Cas12a
Juan Wang1, Fan Zhang2, Ziyang Liu1
1Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, 750004, China. d202081630@alumni.hust.edu.cn.
Abstract:
This study presents a highly sensitive fluorescence biosensor integrating TdT enzyme and CRISPR-Cas12a for precise detection of sperm DNA damage, achieving a detection limit of 0.99 pM and a linear range of 0.001-0.2 nM. Its exceptional sensitivity, simplicity, and versatility make it a transformative tool for reproductive medicine and clinical diagnostics.
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