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Published on: July 11, 2014
Urinary Metabolomic Profile is Minimally Impacted by Common Storage Conditions and Additives
Kelly C Weldon1,2,3, Morgan Panitchpakdi2,3, Andrés Mauricio Caraballo-Rodríguez2,3
1School of Medicine, University of California Davis Health, Sacramento, CA, 95817, USA.
Urine storage conditions do not affect metabolomic analysis. While a DNA stabilizer altered the metabolic profile, parent metabolites remained detectable for accurate human health and disease biomarker discovery.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Biomarker Discovery
Background:
- Metabolomics studies biological system communications.
- Urine is a valuable, noninvasive biofluid for identifying health and disease biomarkers.
- The effects of urine storage and DNA stabilizers on metabolomics are not well understood.
Purpose of the Study:
- To investigate how common storage conditions affect urine metabolomics.
- To assess the impact of the DNA stabilizer AssayAssure® on urine metabolite content.
Main Methods:
- Urine samples were analyzed under various storage conditions.
- The effect of AssayAssure® on urinary metabolite composition was evaluated.
- Metabolomic profiles were compared to assess changes.
Main Results:
- Urinary metabolite profiles were consistent across different storage conditions.
- AssayAssure® addition caused significant metabolic profile alterations via adduct formation.
- Parent metabolite identification and distinction of biological differences were unaffected by AssayAssure®.
Conclusions:
- Urine stored under tested conditions is suitable for metabolomic analysis.
- AssayAssure® does not impede parent metabolite detection, despite altering the overall metabolic profile.
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