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Novel Protein-Rich Bioactive Bioink Stimulates Cellular Proliferation and Response in 3D Bioprinted Volumetric
Suihong Liu1,2,3,4, David Kilian1,5, Anne Bernhardt1
1Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307, Dresden, Germany.
Advanced Healthcare Materials
|February 25, 2025
Summary
Researchers developed a novel, low-cost bioink using eggwhite powder (EWP) to improve 3D bioprinting of functional tissues. This eggwhite powder-supplemented alginate-methylcellulose bioink enhances cell viability and shows promise for tissue engineering applications.
Area of Science:
- Biofabrication
- Tissue Engineering
- Biomaterials Science
Background:
- 3D extrusion bioprinting fabricates tissue substitutes with precise cell distribution.
- Limited bioactive bioinks hinder the creation of functional volumetric tissue substitutes.
- Eggwhite powder (EWP) is an abundant, low-cost protein source.
Purpose of the Study:
- To develop a novel, cost-effective, and bioactive bioink using eggwhite powder (EWP) for 3D bioprinting.
- To enhance the cellular response and printability of alginate-methylcellulose (AlgMC) hydrogels.
- To demonstrate the versatility of the EWP-supplemented bioink for fabricating various tissue types.
Main Methods:
- Functionalization of an alginate-methylcellulose (AlgMC) hydrogel matrix with eggwhite powder (EWP).
- Assessment of bioink printability, shape fidelity, and shear-thinning properties.
- Evaluation of encapsulated cell viability and functionality using stem cells, skin, and bone cells.
- Demonstration of bioprinting strategies including core-shell and multi-channel constructs.
Main Results:
- EWP-supplemented AlgMC bioinks exhibited favorable printability and high shape fidelity.
- Incorporation of EWP enhanced shear-thinning properties, improving cell viability in bioprinted constructs.
- The novel bioink supported three distinct cell types (stem, skin, bone), demonstrating versatility.
- Successful proof-of-concept for functional tissue construction using advanced bioprinting techniques.
Conclusions:
- Eggwhite powder (EWP) is a promising, low-cost additive for creating bioactive bioinks.
- The EWP-supplemented AlgMC bioink significantly enhances cell viability and printability for tissue engineering.
- This novel bioink holds substantial potential for diverse biofabrication and biomedical applications.
