Recent advances in detection techniques for vitamin analysis: A comprehensive review

Area of Science:

• Nutritional Science and Food Chemistry
• Analytical Chemistry and Method Development

Background:

• Vitamins are essential micronutrients critical for human growth and development.
• Vitamins are susceptible to degradation from environmental factors and food processing.
• Dietary insufficiency or malabsorption leads to vitamin deficiencies and associated diseases.

Purpose of the Study:

• To provide a comprehensive evaluation of vitamin detection methods.
• To critically assess analytical techniques for water- and fat-soluble vitamins.
• To review advancements in vitamin analysis over the past five years.

Main Methods:

• Review of microbiology-based analysis.
• Evaluation of spectroscopy and electrochemistry-based techniques.
• Assessment of liquid chromatography-mass spectrometry, sensor, and immunoassay methods.

Main Results:

• Significant advancements in vitamin analysis techniques have been observed.
• Immunoassays demonstrate high sensitivity, simplicity, and affordability for vitamin detection.
• Various methods show promise for accurate quantification of water- and fat-soluble vitamins.

Conclusions:

• Vitamin analysis is critical for nutritional assessment and quality control.
• Immunoassays are a highly effective and practical approach for vitamin quantification.
• Future research should address current challenges and explore new prospects in vitamin analysis.