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Related Experiment Video

Updated: May 26, 2025

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
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Beyond Traditional Lateral Flow Assays: Enhancing Performance Through Multianalytical Strategies.

Eleni Lamprou1, Panagiota M Kalligosfyri1, Despina P Kalogianni1

  • 1Department of Chemistry, University of Patras, Rio, GR26504 Patras, Greece.

Biosensors
|February 25, 2025
PubMed
Summary

Multiplex lateral flow assays (LFAs) offer advanced rapid diagnostics by combining multiple tests. This review highlights their development, applications, and strategies to enhance sensitivity and reliability for future diagnostic innovations.

Keywords:
biosensorimmunoassaymultiplexnanoparticlesnucleic acidspoint-of-care testingrapid testsensorstripvisual detection

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Last Updated: May 26, 2025

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Medical Diagnostics

Background:

  • Multiplex lateral flow assays (LFAs) represent a significant advancement in rapid diagnostics.
  • They integrate multiple tests into a single, user-friendly platform.
  • These assays achieve high specificity and low detection limits through novel strategies.

Purpose of the Study:

  • To review predominant changes in multianalyte LFAs.
  • To discuss their principles, development, and integration with other methods.
  • To highlight strategies for improving assay sensitivity, detectability, and reliability.

Main Methods:

  • Review of current literature on multiplex lateral flow assays.
  • Analysis of novel strategies, including multiple detection labels and amplification methods.
  • Discussion of challenges and solutions in LFA array development.

Main Results:

  • Multiplex LFAs demonstrate versatility across clinical diagnostics, food safety, and environmental monitoring.
  • Advancements focus on enhancing detectability, sensitivity, and reliability.
  • Integration with various methods expands their analytical capabilities.

Conclusions:

  • Multiplex LFAs are crucial for the future of diagnostics and analytical sciences.
  • Continued innovation in LFA technology promises broader applications and improved performance.
  • Addressing challenges in LFA arrays will further unlock their potential.