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Updated: May 24, 2026
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Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Dual Paper-Based Electrochemical Device for Multiplex Detection of Triple-Negative Breast Cancer miRNA Signatures
Alessandra Glovi1,2, Panagiota M Kalligosfyri2, Antonella Miglione3
1Clinical and Translational Oncology, Scuola Superiore Meridionale (SSM), Naples 80138, Italy.
Analytical Chemistry
|May 22, 2026
Summary
This study presents a fast, low-cost paper-based sensor for multiplex microRNA (miRNA) signature analysis. The platform enables simultaneous detection of cancer-related miRNAs in biofluids, offering a promising tool for point-of-care diagnostics.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Molecular Diagnostics
Background:
- Single microRNA (miRNA) detection is insufficient for disease diagnosis.
- miRNA signatures (multiple sequences) provide a more comprehensive reflection of disease biology.
- Accurate and rapid detection of miRNA signatures is crucial for early cancer diagnosis.
Purpose of the Study:
- To develop a paper-based electrochemical sensor for multiplex analysis of cancer-related miRNA signatures.
- To demonstrate the simultaneous detection of specific miRNAs (miRNA-21 and miRNA-101) associated with triple-negative breast cancer.
- To validate the platform's performance in complex biological matrices like human serum.
Main Methods:
- Utilized paper-based screen-printing technology for sensor fabrication.
- Engineered electrodes with capture probes modified with redox mediators (methylene blue and ferrocene) for discrimination.
- Employed electrochemical techniques for simultaneous, interference-free detection of dual miRNA targets.
Main Results:
- Achieved simultaneous detection of miRNA-21 and miRNA-101 within a wide concentration range (0.1-1000 nM).
- Demonstrated clinically relevant low-nanomolar limits of detection in PBS and human serum.
- Exhibited excellent repeatability (RSD ∼4%), rapid assay time (30 min), and reliable detection in spiked serum samples.
Conclusions:
- The developed paper-based platform offers a potent, fast, and low-cost tool for multiplex miRNA signature analysis.
- This technology enables point-of-care diagnostics and can be expanded to larger miRNA panels.
- Highlights the potential for future diagnostic development using multiplex miRNA analysis in biofluids.
