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Published on: September 4, 2013
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Clusterzyme-Enabled Oxidative Stress Alleviation and Microglial Polarization Modulation for Efficient Ischemic Stroke
Lin Zhu1, Weijie Zhong1, Xuchen Meng1
1Department of Neurosurgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
Advanced Healthcare Materials
|February 25, 2025
Summary
New bimetallic nanoclusters (Au7Ag1 NCs) offer neuroprotection against ischemic stroke reperfusion injury by reducing oxidative stress and inflammation. These nanoclusters show promise for treating brain disorders and improving neurological function recovery.
Area of Science:
- Nanomedicine
- Neuroscience
- Biochemistry
Background:
- Ischemic stroke (IS) causes significant global health issues.
- Reperfusion strategies can paradoxically worsen IS neurological damage due to reactive oxygen species (ROS) and neuroinflammation.
Purpose of the Study:
- To develop mercaptosuccinic acid (MSA)-coated bimetallic clusterzymes (Au7Ag1 nanoclusters, NCs) for targeted treatment of IS reperfusion injury.
- To investigate the neuroprotective effects of Au7Ag1 NCs in vitro and in vivo.
Main Methods:
- Synthesis of MSA-coated Au7Ag1 NCs with optimized ratios.
- In vitro and in vivo studies to assess ROS scavenging and neuroinflammation modulation.
- RNA sequencing to analyze molecular pathway impacts.
Main Results:
- Au7Ag1 NCs efficiently scavenge ROS and modulate microglial polarization, reducing oxidative stress.
- Nanoclusters penetrate the blood-brain barrier (BBB) due to ultrafine particle size, enhancing catalytic activity.
- Au7Ag1 NCs reduced brain tissue damage, promoted neurological recovery, and impacted apoptosis and inflammation pathways.
Conclusions:
- Au7Ag1 NCs demonstrate significant neuroprotective potential against IS reperfusion injury.
- This study highlights a promising nanomedicine-based therapeutic strategy for ischemic cerebral disorders.
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