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Immunological markers of peripheral blood and lymph node lymphocytes in Hodgkin's disease
The lymphoid subpopulations of peripheral blood and cell suspensions of lymph nodes were investigated in a series of 20 patients with Hodgkin's disease (HD) by conventional surface markers and a panel of 11 monoclonal antibodies (McAb). In peripheral blood of HD, the number of T lymphocytes and the distribution of helper and suppressor/cytotoxic subpopulations was normal, suggesting that the alteration in cell immunity in this condition is either due to a functional defect in T cells or to an alteration in the immunoregulatory mechanisms which are not directly dependent on T lymphocytes. The lymph nodes involved by HD showed an increase in the number of T helper lymphocytes (OKT4+) as compared with the reactive lymph nodes from 14 subjects used as a control group. In involved lymph nodes there was a good correlation between the number of lymphocytes reacting with the McAb OKT3 and EEAT rosette--forming cells, whereas in most hyperplastic nodes the number of T3 lymphocytes was greater than that of E+ rosettes. Hodgkin and Reed-Sternberg cells did not react either with T, B lineage specific McAb or with myelomonocytic (OKM1) or monocytic (Mo2, FMC-17, FMC-33) McAb. These results would make a monocyte-macrophage lineage origin unlikely also for these cells.
The lymphoid subpopulations of peripheral blood and cell suspensions of lymph nodes were investigated in a series of 20 patients with Hodgkin's disease (HD) by conventional surface markers and a panel of 11 monoclonal antibodies (McAb). In peripheral blood of HD, the number of T lymphocytes and the distribution of helper and suppressor/cytotoxic subpopulations was normal, suggesting that the alteration in cell immunity in this condition is either due to a functional defect in T cells or to an alteration in the immunoregulatory mechanisms which are not directly dependent on T lymphocytes. The lymph nodes involved by HD showed an increase in the number of T helper lymphocytes (OKT4+) as compared with the reactive lymph nodes from 14 subjects used as a control group. In involved lymph nodes there was a good correlation between the number of lymphocytes reacting with the McAb OKT3 and EEAT rosette--forming cells, whereas in most hyperplastic nodes the number of T3 lymphocytes was greater than that of E+ rosettes. Hodgkin and Reed-Sternberg cells did not react either with T, B lineage specific McAb or with myelomonocytic (OKM1) or monocytic (Mo2, FMC-17, FMC-33) McAb. These results would make a monocyte-macrophage lineage origin unlikely also for these cells.