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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

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Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas

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Published on: February 10, 2018

Hempseed Water-Soluble Protein Fraction and Its Hydrolysate Display Different Biological Features.

Annalisa Givonetti¹, Stelvio Tonello², Chiara Cattaneo³

¹Dipartimento per lo Sviluppo Sostenibile e la Transizione Ecologica, Università del Piemonte Orientale, Piazza S. Eusebio 5, 13100 Vercelli, Italy.

Life (Basel, Switzerland)

|February 26, 2025

Summary

Hempseed protein hydrolysates exhibit potent antioxidant activity and do not impact cell viability, unlike native proteins. These findings highlight their potential as functional food ingredients for managing inflammation.

Keywords:

Cannabis sativa IBD MALDI-TOF SDS-PAGE antioxidant autoimmune disease hempseed hempseeds hydrolysates in vitro digestion peptidomics water-soluble protein

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Area of Science:

Food Science
Biochemistry
Nutraceuticals

Background:

Hempseeds (Cannabis sativa) offer a versatile food source with a rich amino acid profile.
Hempseed proteins are being investigated for bioactive peptides with potential health benefits.

Purpose of the Study:

To extract and analyze bioactive peptides from hempseed protein water-soluble fractions.
To evaluate the antioxidant activity and cellular effects of hempseed proteins and peptides.

Main Methods:

Extraction of water-soluble hempseed protein fractions.
Analysis of peptide biological functions, including antioxidant activity (ABTS radical scavenging).
Assessment of protein and peptide impact on U937 cell viability and inflammation modulation.

Main Results:

Hempseed protein hydrolysates demonstrated significantly higher ABTS radical scavenging activity (97.95%) compared to native proteins (81.04%).
Hydrolysates did not affect U937 cell viability, whereas protein treatment significantly reduced it.
Identified peptide bioactivities include ACE inhibition, DPP-IV and DPP-III inhibition, and ubiquitin-mediated proteolysis activation.

Conclusions:

Hempseed-derived peptides possess significant antioxidant properties.
Hempseed hydrolysates show promise as safe functional ingredients for modulating inflammation in gastrointestinal autoimmune diseases.
Further research into hempseed peptides' therapeutic potential is warranted.