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Epigenetic Modulation Directs Recovery Post LASIK and SMILE Surgery: An Experimental Study.

Rohit Shetty¹, Pooja Khamar¹, Ramaraj Kannan²

¹Cornea and Refractive Services, Narayana Nethralaya, Bangalore 560010, India.

Life (Basel, Switzerland)

|February 26, 2025

Summary

This summary is machine-generated.

Refractive surgery like LASIK and SMILE alters DNA methylation in the cornea. These epigenetic changes may contribute to haze complications and suggest new therapeutic targets for better surgical outcomes.

Keywords:

LASIK SMILE epigenetics hypermethylation hypomethylation refractive surgery

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Area of Science:

Ophthalmology
Epigenetics
Corneal Biology

Background:

Refractive surgery (LASIK, SMILE) induces corneal stromal remodeling.
Protein profiles change significantly post-surgery.
DNA methylation status after refractive surgery is largely unknown.

Purpose of the Study:

Investigate epigenetic changes, specifically DNA methylation, following LASIK and SMILE procedures.
Correlate DNA methylation patterns with long-term extracellular matrix remodeling.
Understand the role of DNA methylation in corneal wound healing post-refractive surgery.

Main Methods:

Ex vivo study using 19 donor eye globes.
Performed LASIK and SMILE procedures on separate sets of globes.
Analyzed DNA methylation using the Illumina Infinium Human Methylation 850 EPIC array (HM850) at 3 days and 2 weeks post-surgery.

Main Results:

Significant alterations in DNA methylation patterns were observed in both LASIK and SMILE groups.
Genes related to actin cytoskeleton and inflammation (e.g., smad3, prkca) showed hypomethylation post-LASIK.
Genes involved in sphingolipid metabolism and mucin biosynthesis (e.g., gaa, gstm1) were hypomethylated post-SMILE.

Conclusions:

Altered DNA methylation patterns post-refractive surgery may be linked to haze development.
Findings suggest potential therapeutic strategies involving chromatin remodeling drugs.
Epigenetic modifications offer insights into corneal healing and potential complications.