Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High Intensity Interval Training in Aged Female Mice Preserves Physical, Cognitive, and Cardiovascular Function.

bioRxiv : the preprint server for biology·2026
Same author

TACO1 regulates mitochondrial adaptation in hypertension-induced cardiac remodeling and heart failure.

Research square·2026
Same author

Impact of extreme precipitation events on facility-based births in 21 sub-Saharan African countries.

Nature communications·2026
Same author

PTSD and the Mother-Infant Relationship During the Perinatal Period: A Scoping Review.

Journal of developmental and behavioral pediatrics : JDBP·2026
Same author

A Review of Solid-State LiDAR Principles and Metasurface-Based LiDAR Sensors.

Sensors (Basel, Switzerland)·2026
Same author

The BRD4-nucleosome interaction is enhanced modestly and non-selectively by histone acetylation.

Nucleic acids research·2025

Related Experiment Video

Updated: May 25, 2025

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
10:56

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

Published on: March 26, 2015

21.2K

Relative Quantitation of EFNA1 Expression in Mouse Heart Tissue Histologic Sections Using MALDI-MSI.

Maria Torres1, Laura Gruer2, Smrithi Valsaraj2

  • 1East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC 27834, USA.

International Journal of Molecular Sciences
|February 26, 2025
PubMed
Summary

EphrinA1 (EFNA1) levels decrease after myocardial infarction (MI). A new method using MALDI-MSI quantifies EFNA1 in cardiac tissue, aiding future research into heart disease and treatments.

Keywords:
EFNA1MALDI-MSImyocardial infarction

More Related Videos

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.1K
Noninvasive Assessment of Cardiac Abnormalities in Experimental Autoimmune Myocarditis by Magnetic Resonance Microscopy Imaging in the Mouse
12:24

Noninvasive Assessment of Cardiac Abnormalities in Experimental Autoimmune Myocarditis by Magnetic Resonance Microscopy Imaging in the Mouse

Published on: June 20, 2014

9.9K

Related Experiment Videos

Last Updated: May 25, 2025

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
10:56

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

Published on: March 26, 2015

21.2K
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.1K
Noninvasive Assessment of Cardiac Abnormalities in Experimental Autoimmune Myocarditis by Magnetic Resonance Microscopy Imaging in the Mouse
12:24

Noninvasive Assessment of Cardiac Abnormalities in Experimental Autoimmune Myocarditis by Magnetic Resonance Microscopy Imaging in the Mouse

Published on: June 20, 2014

9.9K

Area of Science:

  • Cardiovascular Biology
  • Proteomics
  • Mass Spectrometry Imaging

Background:

  • EphrinA1 (EFNA1) is a tyrosine kinase receptor-ligand highly expressed in cardiomyocytes, but its levels decrease post-myocardial infarction (MI).
  • Previous studies showed EFNA1-Fc therapy mitigates MI injury by reducing apoptosis, necrosis, and inflammation.
  • Qualitative imaging of endogenous EFNA1 in cardiac tissue post-MI was achieved using MALDI-MSI.

Purpose of the Study:

  • To develop and optimize an integrated quantitative method for determining endogenous EFNA1 levels in cardiac tissue using MALDI-MSI.
  • To characterize the anatomical distribution and relative quantitation of EFNA1 in murine hearts.
  • To establish a foundation for investigating dynamic changes in EFNA1 expression during pathological conditions like MI and in response to therapies.

Main Methods:

  • Optimization of a relative quantitation method for endogenous tryptic EFNA1 peptides in murine heart tissue.
  • Utilized matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) coupled with time-of-flight mass spectrometry (MALDI/TOF MS).
  • Compared the optimized MALDI-MSI method with routine western blotting for EFNA1 quantitation.

Main Results:

  • An optimized method for relative quantitation of endogenous EFNA1 peptides was successfully developed.
  • Quantified approximately 50 ng of EFNA1 per 9.43 mm³ tissue section in healthy myocardium (approx. 12 pg/µg homogenized tissue).
  • MALDI-MSI demonstrated its capability for determining both anatomical distribution and relative quantitation of EFNA1 in cardiac tissue.

Conclusions:

  • MALDI-MSI provides a powerful tool for the anatomical and quantitative assessment of endogenous EFNA1 in cardiac tissue.
  • This quantitative method enables further investigation into EFNA1's role in cardiac pathophysiology, particularly following myocardial infarction.
  • Future applications will facilitate the study of dynamic EFNA1 expression changes in disease states and therapeutic interventions.