Cytotoxicity and Antibacterial Activity of Protonated Diallylammonium Polymers: Influence of End Groups and Molecular...

A series of antimicrobial protonated diallylammonium polymers, poly(diallylammonium trifluoroacetate) (PDAATFA), were synthesized by classical polymerization, using an especially elaborated method for preparation of polymers with low molecular weight (MW), and by RAFT polymerization, with different end groups in a range of MW values of (8-43) × 10³ g∙mol^-1. Cytotoxicity relative to eukaryotic cells (epithelioid lines A-549 and MA-104) and bactericidal activity of the polymers (relative to Pseudomonas aeruginosa and Staphylococcus aureus) are investigated. The effect of the end groups and MW on toxicity and bactericidal activity is shown. Dependence of the activity and, most of all, cytotoxicity on MW is preserved even at a small difference in MW values in the MW range of (18-40) × 10³ g·mol^-1. A clear dependence of the studied properties on the nature of the terminal group is revealed. Sulfate -O-S(=O)₂-O¯ end group has a noticeable effect on the bactericidal efficiency and smaller influence on toxicity, while dithiocarbonyl end group -S-C(=S)-O-CH₂-CH₃ has a significant effect on efficiency and especially toxicity, drastically increasing the latter. Overall, based on the results obtained, polymers PDAATFA of low MW are considered promising antimicrobial agents for the creation of new transdermal drugs.