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Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Determination of the Polyphenol Composition of Raspberry Leaf Using LC-MS/MS.

Hind Mesfer S Alkhudaydi1,2, Esther Njeri Muriuki1, Jeremy P E Spencer1

  • 1Department of Food and Nutritional Sciences, School of Chemistry, Food and Pharmacy, University of Reading, Reading RG6 6DZ, UK.

Molecules (Basel, Switzerland)
|February 26, 2025
PubMed
Summary

Raspberry leaf (RL) is rich in polyphenols, especially ellagitannins and ellagic acid. Optimal brewing for tea extraction is around 5 minutes to maximize polyphenol absorption.

Keywords:
LC-MSpolyphenolic profileprincipal component analysisraspberry leaf

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Area of Science:

  • Phytochemistry
  • Food Science
  • Nutraceuticals

Background:

  • Raspberry leaf (RL) is a cultivation by-product with potential bioactive compounds.
  • Polyphenolic composition of RL requires further extensive study.

Purpose of the Study:

  • Evaluate (poly)phenolic content in six RL samples from diverse locations.
  • Determine the impact of brewing duration on accessible phenolic compounds.

Main Methods:

  • Utilized Liquid Chromatography-Mass Spectrometry (LC-MS) to analyze 52 polyphenolic constituents.
  • Extracted RL tea using methanol/acidified water with sonication.
  • Brewed RL tea (2 g/200 mL) at various times (0.5-20 min) to assess polyphenol release.

Main Results:

  • Total polyphenol content ranged from 358.66 to 601.65 mg/100 g across samples.
  • Ellagitannins were predominant (155.27–394.22 mg/100 g), followed by flavonoids and phenolic acids.
  • Maximum polyphenol yield (505.65 mg/100 g) occurred at 5 min brewing time, with ellagic acid peaking at this time.

Conclusions:

  • RL possesses a significant concentration of polyphenols, notably ellagic acid.
  • Consumption of RL may enhance daily intake of beneficial non-nutrient compounds.
  • Optimal steeping time of 5 minutes maximizes polyphenol extraction from RL tea.