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Updated: May 25, 2025
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Author Spotlight: Investigating Wound Healing in Mice Models of Oronasal Fistulas
Published on: September 8, 2023
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Microbial Changes Occurring During Oronasal Fistula Wound Healing
Steven L Goudy1,2,3, Heath L Bradley1,2, Camilo Anthony Gacasan2
1Department of Otolaryngology, Emory University, Children's Healthcare of Atlanta, Atlanta, GA 30329, USA.
Microorganisms
|February 26, 2025
Summary
An oro-nasal fistula (ONF) in mice disrupts the oral microbiome, causing pathogen blooms that may impede healing. Therapeutics targeting ONF healing could restore microbiome balance and prevent pathogen overgrowth.
Area of Science:
- Microbiology
- Wound Healing
- Oral Health
Background:
- The oral microbiome is complex and linked to systemic health.
- Superficial oral wounds heal efficiently, but oro-nasal fistulas (ONFs) present a healing challenge.
- ONFs often result from cleft palate surgery.
Purpose of the Study:
- To investigate oral microbiome alterations following ONF creation in mice.
- To understand the impact of ONF on wound healing and microbial composition.
Main Methods:
- Characterized oral microbiome changes in mice with induced ONFs.
- Administered oral antibiotics pre-ONF infliction to assess microbial and healing impacts.
Main Results:
- ONF creation reduced oral microbiome diversity and led to blooms of *Enterococcus faecalis*, *Staphylococcus lentus*, and *Staphylococcus xylosus*.
- Antibiotic treatment before ONF infliction reduced microbial diversity and prevented pathogen blooms but did not improve ONF healing.
- The presence of an ONF created a dysbiotic oral microbiome with opportunistic pathogen blooms.
Conclusions:
- ONFs in mice induce oral dysbiosis and opportunistic pathogen blooms, potentially hindering healing.
- Therapeutic strategies accelerating ONF healing may restore oral microbiome diversity and control pathogen overgrowth.
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