Engineering gold nanoclusters anchored cobalt oxyhydroxide with bifunction for discriminative monitoring
Shun Wang1, Guoming Sun2, Yanzhao Wang3
1Application Center for Precision Medicine, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; College of Medical Engineering, Jining Medical University, Jining 272067, China.
Abstract:
Precise and convenient detection of azodicarbonamide (ADC) is indispensable for safeguarding food safety and public health. Herein, a new ratio fluorescence strategy was strategically designed for monitoring ADC with excellent sensitivity and strong resistance to interference based on gold nanoclusters anchored cobalt oxyhydroxide nanocomposite (AuNCs@CoOOH). The AuNCs@CoOOH integrated two functions of CoOOH (quencher and mimetic oxidase) and fluorescence property of AuNCs, which can catalyze oxidation of non-fluorescent thiamine to fluorescent thiochrome. Meanwhile, the fluorescence emitted by AuNCs was attenuated by CoOOH via a fluorescence resonance energy transfer, forming a ratio response. Owing to analyte-initiated reduction of CoOOH, the AuNCs@CoOOH recognized glutathione. Coupling with specific reactions of ADC and glutathione, a ratio-based fluorescent platform is devised for sensitive detection of ADC, achieving a detection limit of 0.034 μM. The method proved an effective way for accurately monitoring ADC in flour samples, thereby enhancing its practical utility in ensuring food safety.
