Related Experiment Video
Updated: May 25, 2025

Establishment of a Mouse Severe Acute Pancreatitis Model using Retrograde Injection of Sodium Taurocholate into the Biliopancreatic Duct
Published on: April 1, 2022
Pentraxin3 exacerbates acute pancreatitis injury by inhibiting oxidative phosphorylation pathway
Wenyue Wang1,2, Yaning Wang1, Chenchen Yuan1,2
1Pancreatic Center, Department of Gastroenterology, Yangzhou Key Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu, China.
Abstract:
Local pancreatic necrosis and systemic inflammatory response caused by acute pancreatitis (AP) are closely related to the disease prognosis and severity. This study aimed to explore whether pentraxin 3 (PTX3) regulates AP pancreatic necrosis and reveals the underlying mechanism. By using AP time gradient transcriptomics, proteomics and liquid phase chip analysis, we found a close association between PTX3 and AP. Subsequently, caerulein (CAE) induced in vivo AP model and CCK induced in vitro acinar cell damage model were constructed to determine the expression of PTX3 and its regulation of AP. The results showed that PTX3 was highly expressed in the CAE-induced AP model. And more severe pancreatic tissue damage and increased serum amylase were observed after the intervention of recombinant protein PTX3, which was strongly linked to the decreasing in mitochondrial membrane potential, increasing in reactive oxygen species, and regulation by oxidative phosphorylation pathway. PTX3 could exacerbate acinar cell damage in AP by mediating the process of oxidative phosphorylation.
Related Concept Videos
Acute Pancreatitis I: Introduction
Acute pancreatitis is characterized by rapid inflammation of the pancreas, often caused by factors like gallstone blockage or excessive alcohol consumption. Chronic pancreatitis, on the other hand, is a slow, progressive inflammation that may result from long-term alcohol abuse, obstructions in the pancreatic duct, or genetic factors.
The causes of acute pancreatitis include:
Chronic Pancreatitis I: Introduction
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
Acute Pancreatitis II: Clinical Manifestations and Management
Chronic Pancreatitis II: Collaborative Care
Assessment:
The Electron Transport Chain
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...

