Related Experiment Video
Updated: May 25, 2025

CRISPR Guide RNA Cloning for Mammalian Systems
Published on: October 2, 2018
Genome-wide CRISPR guide RNA design and specificity analysis with GuideScan2
Henri Schmidt1,2, Minsi Zhang3, Dimitar Chakarov4
1Department of Computer Science, Princeton University, Princeton, NJ, USA.
None:
We present GuideScan2 for memory-efficient, parallelizable construction of high-specificity CRISPR guide RNA (gRNA) databases and user-friendly design and analysis of individual gRNAs and gRNA libraries for targeting coding and non-coding regions in custom genomes. GuideScan2 analysis identifies widespread confounding effects of low-specificity gRNAs in published CRISPR screens and enables construction of a gRNA library that reduces off-target effects in a gene essentiality screen. GuideScan2 also enables the design and experimental validation of allele-specific gRNAs in a hybrid mouse genome. GuideScan2 will facilitate CRISPR experiments across a wide range of applications.
Related Concept Videos
CRISPR
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

